Discrepancies between results obtained by agar and broth techniques in testing of drug combinations.
نویسندگان
چکیده
One hundred strains of Staphylococcus epidermidis were tested by the double-disk agar diffusion method for their susceptibility to cephalothin, to sulfamethoxazole-trimethoprim, and to combinations of these drugs. Forty-five strains showed a bridging inhibition zone between the two disks, suggesting a synergistic effect of the combination of cephalothin plus sulfamethoxozole-trimethoprim against these strains. Ten of these strains were retested against the individual drugs and their combination by the twofold broth dilution method. No strain showed higher susceptibility to any combination as compared with cephalothin alone.
منابع مشابه
Comparison of five methods, including the PDM Epsilometer test (E test), for antimicrobial susceptibility testing of Pseudomonas aeruginosa.
The antimicrobial susceptibilities of 100 clinical isolates of Pseudomonas aeruginosa to six antipseudomonal antibiotics were tested by five methods: the National Committee for Clinical Laboratory Standards (NCCLS) methods for broth microdilution, agar dilution, and agar disk diffusion; the Vitek Automicrobic System method (Vitek Systems, Hazelwood, Mo.); and the PDM Epsilometer test (E test) (...
متن کاملIn Vitro Activities of b-Lactam–b-Lactamase Inhibitor Combinations against Stenotrophomonas maltophilia: Correlation between Methods for Testing Inhibitory Activity, Time-Kill Curves, and Bactericidal Activity
The activities of ampicillin, ampicillin-sulbactam, amoxicillin, amoxicillin-clavulanic acid, ticarcillin, ticarcillin-clavulanic acid, piperacillin, piperacillin-tazobactam, aztreonam, and aztreonam-clavulanic against Stenotrophomonas maltophilia strains for which the MICs of penicillins and commercially available b-lactam– b-lactamase inhibitor combinations were higher than the breakpoints us...
متن کاملComparison of BSAC agar dilution and NCCLS broth microdilution MIC methods for in vitro susceptibility testing of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis: the BSAC Respiratory Resistance Surveillance Programme.
OBJECTIVE The aim of this study was to establish the degree of comparability between the NCCLS broth microdilution and BSAC agar dilution MIC methods of antimicrobial susceptibility testing. METHODS Six hundred and sixty-one clinical isolates of Streptococcus pneumoniae, 936 Haemophilus influenzae and 421 Moraxella catarrhalis, collected in the winter of 1999-2000 by 20 laboratories in the UK...
متن کاملPractical anaerobic broth-disk elution susceptibility test.
A modified broth-disk elution method for routine susceptibility testing of anaerobic bacteria was compared with the proposed reference agar dilution method under study by the National Committee on Clinical Laboratory Standards. The broth-disk elution method used a 24-h test incubation period, Schaedler broth medium supplemented with menadione, and GasPak (BBL Microbiology Systems) incubation. R...
متن کاملEvaluation of in vitro methods for testing ceftriaxone against anaerobic bacteria, including quality control guidelines.
Tests with 100 anaerobic bacterial isolates demonstrated comparability between ceftriaxone MICs obtained with the reference agar dilution procedure and those obtained with a broth microdilution susceptibility testing procedure. The aerobically incubated thioglycolate disk elution test was also evaluated. Six 30-micrograms ceftriaxone disks in 5 ml of thioglycolate separated strains for which MI...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 11 5 شماره
صفحات -
تاریخ انتشار 1980